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Image Search Results
Journal: Journal of Experimental & Clinical Cancer Research : CR
Article Title: Gli1-mediated tumor cell-derived bFGF promotes tumor angiogenesis and pericyte coverage in non-small cell lung cancer
doi: 10.1186/s13046-024-03003-0
Figure Lengend Snippet: The CM of Gli1-overexpressing NSCLC cells promoted angiogenesis in vitro and ex vivo. A Flowchart of evaluation the effect of Gli1-overexpressing A549 and NCI-H460 cells on endothelial cell and aorta rings. B-E The effect of CM from A549 Gli1 vector and NCI-H460 Gli1 vector cells on the migration, invasion and tube formation abilities of HUVECs and HMEC-1 cells. HUVECs and HMEC-1 cells were treated with CM for 48 h and subjected for wound healing ( B ), Transwell migration ( C ) and Tiranswell invasion ( D ), and tube formation assays ( E ). F Gli1-overexpressing NSCLC increased blood sprouting in aortic ring assay. G The CM of Gli1-overexpressing cells promoted the HBVP adhesion ability. H Schematic strategy of coculture tubule-like structure of endothelial cells and pericytes. I CM from Gli1-overexpressing cells enhanced the recruitment of HBVP to tubes formed by endothelial cell. All the data were showed as mean ± SD, n = 3. ** p < 0.01; *** p < 0.001 vs A549 NC vector or NCI-H460 NC vector cells
Article Snippet:
Techniques: In Vitro, Ex Vivo, Plasmid Preparation, Migration, Aortic Ring Assay
Journal: Journal of Experimental & Clinical Cancer Research : CR
Article Title: Gli1-mediated tumor cell-derived bFGF promotes tumor angiogenesis and pericyte coverage in non-small cell lung cancer
doi: 10.1186/s13046-024-03003-0
Figure Lengend Snippet: The CM from Gli1-knockdown NSCLC cells suppressed angiogenesis in vitro and ex vivo. A Schematic strategy of endothelial cells and aorta rings treated with CM from Gli1-knockdown NSCLC cells. B-E The CM from NCI-H1299 shGli1 cells or NCI-H1703 shGli1 cells suppressed the migration ( B and C ), invasion ( D ) and tube formation ( E ) abilities of HUVECs and HMEC-1 cell. F The CM from NCI-H1299 shGli1 cells or NCI-H1703 shGli1 cells reduced vessel sprouting in the rat aorta rings. G-I The CM from NCI-H1299 shGli1 cells and NCI-H1703 shGli1 cells inhibited HBVP adhesion ( G ) and recruitment to newly blood vessel (H and I). All the data were showed as mean ± SD, n = 3. ** p < 0.01; *** p < 0.001 vs NCI-H1299 sh NC or NCI-H1703 sh NC cells
Article Snippet:
Techniques: Knockdown, In Vitro, Ex Vivo, Migration
Journal: ACS Applied Materials & Interfaces
Article Title: Insights into the Mechanism of Supramolecular Self-Assembly in the Astragalus membranaceus – Angelica sinensis Codecoction
doi: 10.1021/acsami.3c09494
Figure Lengend Snippet: AA-NPs inhibited the EndMT process in HUVECs and HMEC-1 in vitro. After Ang II stimulation, the expression levels of collagen-I, fibronectin, CD31, α-SMA, and FSP-1 proteins in (A) HUVECs and (B) HMEC-1 treated with different concentrations of AA-NPs. (C) Morphological changes of HUVECs observed under a microscope before and after AA-NPs treatment. (D) Immunofluorescence staining of the mesenchymal marker α-SMA and vascular endothelial marker CD31 in HUVECs. All data are presented as the mean ± SD ( n = 3).
Article Snippet:
Techniques: In Vitro, Expressing, Microscopy, Immunofluorescence, Staining, Marker